Phorbol Esters Stimulate Ubiquitination of NCC By Activating ERK1/2
نویسندگان
چکیده
منابع مشابه
Tumor-promoting phorbol esters stimulate hematopoietic colony formation in vitro.
Tumor-promoting phorbol esters stimulated mouse bone marrow cells to form myeloid colonies in agar cultures without added colony-stimulating factors. The colony-stimulating ability of various phorbol esters correlated well with their ability to promote skin tumors in vivo. These results suggest that phorbol esters mimic the action of specific colony-stimulating factors that regulate growth.
متن کاملPhorbol esters stimulate macropinocytosis and solute flow through macrophages.
The morphology and kinetics of pinocytosis by bone marrow-derived macrophages were studied to determine how stimulation by phorbol esters increases net solute accumulation. Application of phorbol myristate acetate (PMA) increased both the abundance of macropinosomes and the rate of solute flow through the endocytic compartment. The large pinosomes originated as ruffles at the cell margins that ...
متن کاملRasGRP1 stimulation enhances ubiquitination and endocytosis of the sodium-chloride cotransporter.
The sodium-chloride cotransporter (NCC) is the principal salt-absorptive pathway in the distal convoluted tubule. Recently, we described a novel pathway of NCC regulation in which phorbol esters (PE) stimulate Ras guanyl-releasing protein 1 (RasGRP1), triggering a cascade ultimately activating ERK1/2 MAPK and decreasing NCC cell surface expression (Ko B, Joshi LM, Cooke LL, Vazquez N, Musch MW,...
متن کاملTumor-promoting Phorbol Esters
The effect of tumor-promoting phorbol esters on the in vitro proliferation of mouse pluripotent hematopoietic stem cells (CFU-S) was examined using a short-term in vitro culture system and an 11-d spleen colony assay. Phorbol myristate acetate (PMA, 10-7 M), but not the inert compound phorbol, supported the in vitro survival of day 11 CFU-S for 72 h in a manner similar to IL 3. PMA also enhance...
متن کاملMembrane effects of phorbol esters.
SUMMARY Tetradecanoyl-phorbol-acetate (TPA) was observed to produce a 15% decrease in the electrophoretic mobility of hyperdiploid Ehrlich-Lettré ascites tumor cells (ELD). This reduction in mobility of ELD was capable of being reversed by washing the cells in Ca2@-free Krebs-Ringer phosphate. An inactive phorbol stereoisomer (4a-phorbol-didecanoate) did not reduce the mobility of ELD. Effects...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2009
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.23.1_supplement.796.38